Dodt gradient contrast microscopy | Published in BioPhotonics

Imaging transparent, colorless specimens has always presented a challenge in optical microscopy. In many applications, it is not desirable or even possible to use fluorescent dyes, stains or transgenic probes to render target objects more visible.  While differential image contrast (DIC), often in the form of the Nomarski microscope, is widely employed, its optical complexity can cause limitations when the contrast image is combined with some other measurement modality or imaging technique (such as patch-clamp measurements or optogenetic stimulation, or even simply epi-fluorescence)..... Biophotonics 2017-11 Microscopy