Originally designed to pull
patch-recording electrodes, the improved EP-450 can now pull a wide verity
of electrode shapes, including sharp electrodes for microinjection and
intracellular experiments. This dual stage pipette puller uses
several unique methods to make consistent electrodes pull after pull.
The theory of operation is based on time management and milli-voltage
control of the element for both first and second pulls. By
controlling the time, or at least establishing a time basis, the user can
create a pipette shape and length consistently and repeatedly.
The EP-450 has been designed to be
multi-user friendly. A simple data log book is included to record
electrode glass type, electrode glass diameter, first pull time, first
pull voltage, dwell time, pipette symmetry, pull tension, second pull time
and second pull voltage. Using this information, any user can easily
reestablish their settings and recreate a specific electrode shape.
The EP-450 has a unique ergonomic design. It is comfortable for the
user to operate on a daily basis. It has been constructed for easy
access to the loading mechanism, which eliminates operator bending and
kneeling. All controls are set at a 45° angle for comfortable
viewing.
A fine pitch 100TPI pipette stop
adjustment screw makes it very easy to balance the upper and lower
electrodes to ensure symmetric electrode lengths every pull. The
filament carriage travels from the first pull location to the second pull
location and has a micrometer dial read-out for fine adjustment. The
carriage also has a simple heat shield that uses a standard 25mm x 75mm
microscope slide to protect the element during the pull process. For
more details, contact Siskiyou or see our web site at www.siskiyou.com
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